Kook Han Kim
hani5736@skku.edu


Oct. 2006 - Feb. 2011
Ph.D. Biotechnology and Genetic Engineering, Korea Univ., Seoul, Korea
Oct. 2003 - Sep. 2005
M.S. Life Sciences and Biotechnology, Korea Univ., Seoul, Korea
Mar. 1995 - Sep. 2002
B.S. Microbiology, Chung Nam Univ., Daejoen, Korea


Publications

1. Lee WK , Son SH, Jin BS, Na JH, Kim SY, Kim KH, Kim EE, Yu YG, Lee HH. Structural and functional insights into the regulation mechanism of CK2 by IP6 and the intrinsically disordered protein Nopp140. Proc. Natl. Acad. Sci. U S A. 2013, Nov 26;110(48):19360-5.

2. Kim KH, Ahn HJ, Lee WK, Lee C, Yu MH, Kim EE. Novel β-structure of YLR301w from Saccharomyces cerevisiae. Acta Crystallogr D Biol Crystallogr. 2012, May;68(Pt 5):531-40. Impact factor=14.

3. Kim KH, Ha BH, Kim SJ, Hong SK, Hwang KY, Kim EE. Crystal structures of Enoyl-ACP reductases I (FabI) and III (FabL) from B. subtilis. J Mol Biol. 2011, Feb 25;406(3):403-15.

4. Kim SJ, Ha BH, Kim KH, Hong SK, Shin KJ, Suh SW, Kim EE. Dimeric and tetrameric forms of enoyl-acyl carrier protein reductase from Bacillus cereus. Biochem Biophys Res Commun. 2010, Oct 1;400(4):517-22.

5. Hong SK, Kim KH, Park JK, Jeong KW, Kim Y, Kim EE. New design platform for malonyl-CoA-acyl carrier protein transacylase. FEBS Lett. 2010, Mar 19;584(6):1240-4.

6. Hong SK, Kim KH, Kim EE. Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010, Jan 1;66(Pt 1):20-2.

7. Han KC, Kim JH, Kim KH, Kim EE, Seo JH, Yang EG. Identification of farnesoid X receptor modulators by a fluorescence polarization-based interaction assay. Anal Biochem. 2010, Mar 15;398(2):185-90.

8. Nam KH, Kim KH, Kim EE, Hwang KY. Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing. Biochem Biophys Res Commun. 2009, Apr 17;381(4):630-3.

9. Kim KH, Jung EJ, Im H, Lelie DV, Kim EE. Expression, purification, and crystallization and preliminary X-ray crystallographic analysis of CnrX from Cupriavidus metallidurans CH34. J Microbiol Biotechnol. 2008, Jan;18(1):43-7.

10. Park JK, Kim KH, Moon JH, Kim EE. Characterization of peptide deformylase2 from B. cereus. J Biochem Mol Biol. 2007, Nov 30;40(6):1050-7.

11. Kim KH*, Bong YJ*, Park JK, Shin KJ, Hwang KY, Kim EE. Structural basis for glutamate racemase inhibition. J Mol Biol. 2007, Sep 14;372(2):434-43.

12. Kim KH, Park JK, Ha BH, Moon JH, Kim EE. Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007, Mar 1;63(Pt 3):246-8.


Research Experience

Mar.2011 - Apr.2014 
 Postdoctoral Fellow, Korea Institute of Science and Technology (KIST) Biomedical Research Institute

Oct. 2005 - Sep. 2006
 Research scientist, Korea Institute of Science and Technology (KIST) Life Sciences Division


Research interests 


 Structural basis of drug candidates and its application for the drug development

· Structure determination and analysis of diseases target protein with its ligand

· Therapeutic agent development by SBDD (Structure-Based Drug Design) of antibiotics, anti-cancer, anti-inflammatory agents

 Crystallographic & dynamics studies of biological macromolecules

· Proteins associated with cell signaling or cell damage

· Ubiquitination and deubiquitination enzymes for regulation of a variety of cellular process, including cell proliferation, differentiation, and death.

 Structure and function of cellular membrane protein and compartments

 Stress related protein and its signal transduction mechanism, and transcriptional regulation


Technical Skills

 Proficient in cloning, protein expression and purification, structural biology using X-ray crystrallography, isothermal titration calorimetry.


Honors and Awards

Aug.10 2007
 Scholarship for Excellence in Research, Korea Institute of Science and Technology

Nov.22 2012 
 Rising Scholarship Award, Korean Synchrotron Radiation User’s Association



Presentations

1. Hong SK, Kim KH, Hwang KY, Kim EE. Crystal Structure of IUS-SPRY Domain from RanBPM/10 Family. Korean Crystallographic Association Meeting. Nov 1, 2013, Seoul, Korea.

2. Hong SK, Kim KH, Hwang KY, Kim EE. Structure of IUS-SPRY domain from RanBPM and RanBP10. International Conference of the Korean Society for Molecular and Cellular Biology. Oct 11, 2013, Seoul, Korea.

3. Hong SK, Kim KH, Hwang KY, Kim EE. Structural Analysis of SPRY/B30.2 Domain Proteins. International Conference of the Korean Society for Molecular and Cellular Biology. Oct 11, 2013, Seoul, Korea.

4. Kim KH, Kim EE. Crystal Structure of the Metal-Sensor CnrX from Cupriavidus metallidurans CH34. The 24th Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 12, 2012, Seoul, Korea.

5. Shin SC, Lee JH, Kim KH, Park JK, Keum GC, Kim EE. Structural and Thermodynamic Characterization of 2-Aminopurine Derivatives as Heat Shock Protein 90 Inhibitors. The 24th Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 12, 2012, Seoul, Korea.

6. Kim KH, Anh HJ, Lee WK, Lee CJ, Yu MH, Kim EE. Dimeric Structure of YLR301w from Saccharomycces. The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 6, 2011, Seoul, Korea.

7. Shin SC, Kim KH, Park JK, Keum GC, Kim EE. Binding of BIIB-021 and 17AAG to HSP90 and GRP94. The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 6, 2011, Seoul, Korea.

8. Kim SJ, Kim KH, Suh SW, Kim EE. Dimeric and Tetrameric forms of enoyl-ACP reductase. XXII congress and General Assembly of the International Union of Crystallography. Aug 25, 2011, Madrid, Spain.

9. Shin SC, Kim KH, Park JK, Keum GC, Kim CW, Kim EE. BIIB-021 Binding to th N-terminal domain of Hsp90-structural and Thermodynamic Characterization. The 22nd Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 8, 2010, Seoul, Korea.

10. Kim KH, Kim SJ, Hong SK, Kim EE. Structural comparison for Enoyl-ACP Reductase I(FabI) and III(FabL) from Bacillus subtilis. The 22nd Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 8, 2010, Seoul, Korea.

11. Park JK, Kim KH, Kim EE. Molecular insights of p97 and adaptor protein fas-associated factor 1. 2010 KSBMB Annual Meeting. May 18, 2010, Seoul, Korea.

12. Kim SJ, Kim KH, Ha BH, Shin KJ, Suh SW, Kim EE. Cyrstal structures of bacillus cereus enoyl-acyl carrier protein reductase in free and complexed to nadp+ and an indole naphthyridinone. 2010 KSBMB Annual Meeting. May 17, 2010, Seoul, Korea.

13. Hong SK, Kim KH, Park JK, Kim YM, Kim EE. New Design Platform for Malonyl-CoA-Acyl Carrier Protein Transacylase. The 21th Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 16, 2009, Seoul, Korea.

14. Kim KH, Jung EJ, Im H, Daniel L, Kim EE. Expression, purification, and crystallization and preliminary X-ray crystallographic analysis of CnrX from Cupriavidusmetallidurans CH34. Korean Crystallographic Association Meeting. May 23, 2008, Seoul, Korea.

15. Hong SK, Kim KH, Park JK, Chong YH, Kim JH, Kim EE. Structure of BsFabF/Cerulenin Complex. The 19th Annual Meeting of the Korean Society for Molecular and Cellular Biology. Oct 18, 2007, Seoul, Korea.

16. Park JK, Kim KH, Moon JH, Kim EE. Characterization of PDF2 from Bacillus cereus. 19th FAOBMB Seoul Conference. May 28, 2007, Seoul, Korea.

17. Kim KH, Park JK, Ha BH, Moon JH, Kim EE. Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis. 19th FAOBMB Seoul Conference. May 28, 2007, Seoul, Korea.

18. Kim KH, Bong YJ, Park JK, Shin KJ, Hwang KY, Kim EE. Structural Basis for Glutamate Racemase Inhibition. 19th FAOBMB Seoul Conference. May 28, 2007, Seoul, Korea.

19. Kim KH, Moon JH, Lee HK, Kim IY, Kim EE. Structural Studies of Actinonin Bound Peptide Deformylases and Implication in Drug Design. 5th International Symposium on Antimicrobial Agents and Resistance. April 28, 2005, Seoul, Korea.

Deposited Structures

 - Novel fold & unknown function : 3RBY
 - Enoyl-ACP reductase : 3OIC, 3OID, 3OIF, 3OIG, 3OJE, 3OJF
 - Peptide deformylase : 2OS0, 2OS1, 2OS3
 - Glutamate racemase : 2OHO, 2OHV, 2OHG
 - Fas-associated factor 1 : 3QWZ, 3QX1
 - Malonyl acyl carrier protein transacylase : 3IM8, 3IM9
 - Ran binding protein 9 (RanBPM) : 4ORP, 4ORQ
 - Ran binding protein 10 : 4ORT 
 - Muskelin : 4PQQ